Genetically recombinant Saccharomyces cerevisiae for degrading kitchen waste

US10584359B2 · kind B2 · utility

Assignee

• Guangdong Recyclean Low-Carbon Technology · CN

Inventors

• Zehuan LIU · Tangxia, CN
• Long Fang · Zhejiang, CN
• Kai Yan · Beijing, CN
• Xiaolong Kang · Tangxia, CN
• Yangyang Zheng · Tangxia, CN
• Renhuai Liu · Tangxia, CN
• Jianghai LIN · Tangxia, CN
• Wenjuan Xiao · Tangxia, CN
• Jingbo LI · Nanjing, CN
• Yingxue Gong · Tangxia, CN

Key dates

• Filing date: Mar 21, 2014
• Grant date: Mar 10, 2020
• Expiry date: Jun 24, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is a genetically recombinant Saccharomyces cerevisiae useful for degrading and utilizing kitchen wastes. Genes encoding α-amylase (AMY), glucoamylase (GA) and acid protease (AP) were introduced into the genetically recombinant Saccharomyces cerevisiae using a Saccharomyces cerevisiae multi-gene co-expression vector and successfully expressed and secreted. The Saccharomyces cerevisiae so obtained are capable of secreting amylases and protease to degrade the starch and proteins in kitchen wastes to produce carbon and nitrogen sources such as glucose, polypeptides and amino acids, allowing fermentation into ethanol.