Method of nucleic acid sequence determination
US10584379B2 · kind B2 · utility
4Cited by
16References
17Claims
0Family size
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Key dates
| Filing date | Apr 28, 2017 |
| Grant date | Mar 10, 2020 |
| Priority date | — |
| Expiry date | May 31, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.