Systems and methods for biomimetic fluid processing

US10590373B2 · kind B2 · utility

Assignees

• The Brigham and Women's Hospital, Inc. · US
• President and Fellows of Harvard College · US
• Vilnius University · LT

Inventors

• Jonathan N. Thon · Brookline, US
• Joseph Italiano · Sharon, US
• Linas Mazutis · Vilnius, LT
• David A. Weitz · Bolton, US

Key dates

• Filing date: Mar 30, 2015
• Grant date: Mar 17, 2020
• Expiry date: Nov 17, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2521/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.