Methods of preventing and removing trisulfide bonds
US10590454B2 · kind B2 · utility
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12References
13Claims
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Key dates
| Filing date | Oct 16, 2017 |
| Grant date | Mar 17, 2020 |
| Priority date | — |
| Expiry date | Oct 16, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/34
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.