Patent · US Active

Methods for true isothermal strand displacement amplification

US10590474B2 · kind B2 · utility

0Cited by

22References

16Claims

0Family size

Assignee

ELITECHGROUP B.V. · NL

Inventors

Yevgeniy S. Belousov · Mill Creek, US
Boris Alabeyev · Lynnwood, US
Noah Scarr · Seattle, US

Key dates

Filing date	Dec 20, 2017
Grant date	Mar 17, 2020
Priority date	—
Expiry date	Aug 8, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6823
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods, primers and probes are provided for the isothermal amplification and detection, without denaturation, of double stranded nucleic acid targets for polymerase strand displacement amplification (“iSDA”). The methods and compositions disclosed are highly specific for nucleic acid targets with high sensitivity, specificity and speed that allow detection of clinical relevant target levels. The methods and compositions can easily be used to amplify or detect nucleic acid targets in biological samples.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.