Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
US10590477B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 2017 |
| Grant date | Mar 17, 2020 |
| Priority date | — |
| Expiry date | Apr 28, 2038 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0409
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.