Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
US10591486B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 21, 2015 |
| Grant date | Mar 17, 2020 |
| Priority date | — |
| Expiry date | Nov 27, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/47
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting an interaction between one or more protein bait and one or more candidate prey in a eukaryotic cell, comprising the steps of: a) providing an eukaryotic cell expressing (i) one or more protein bait, and (ii) one or more candidate prey, wherein said protein bait comprises a bait moiety and a polymerized-tubulin binding moiety. b) determining the occurence of an interaction between said one or more protein bait and said one or more candidate prey in the eukaryotic cell, wherein said protein bait is bound to polymerized tubulin in the eukaryotic cell, thereby localizing said one or more candidate prey along said polymerized tubulin, thereby detecting said interaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.