Imaging platform based on nonlinear optical microscopy for rapid scanning large areas of tissue
US10595770B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 17, 2017 |
| Grant date | Mar 24, 2020 |
| Priority date | — |
| Expiry date | Aug 11, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B2207/114
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multiphoton microscope based on two-photon excited fluorescence and second-harmonic generation that images FOVs of about 0.8 mm2 (without stitching adjacent FOVs) at speeds of 10 frames/second (800×800 pixels) with lateral and axial resolutions of 0.5 μm and 2.5 μm, respectively. The scan head of the instrument includes a fast galvanometric scanner, relay optics, a beam expander and a high NA objective lens. The system is based on a 25×, 1.05 NA water immersion lens, which features a long working distance of 1 mm. A proper tailoring of the beam expander, which consists of the scan and tube lens elements, enables scaling of the FOV. The system and method also include a flat wavefront of the beam, minimum field curvature, and suppressed spherical aberrations. All aberrations in focus are below the Marechal criterion of 0.07λ rms for diffraction-limited performance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.