Animal product-free culture medium for bacteria of the genus Clostridium and a process for producing supernatant comprising one or more collagenolytic and gelatinolytic proteases
US10597632B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 14, 2017 |
| Grant date | Mar 24, 2020 |
| Priority date | — |
| Expiry date | Jul 29, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/24003
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention refers to a culture medium and a process for producing proteases with collagenolytic and gelatinolytic activity by bacteria of the genus Clostridium. Particularly, the present invention refers to an animal product-free culture medium for C. histolyticum, characterized by comprising non-animal origin peptones, preferably vegetable peptones, yeast extract and the amino acids cysteine and arginine. The present invention also refers to a process for producing Clostridium histolyticum liquid culture supernatant comprising one or more proteases with collagenolytic and gelatinolytic activity, and pharmaceutical composition comprising as active ingredient the supernatant or the supernatant optionality purified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.