Polymerases engineered to reduce nucleotide-independent DNA binding

US10597643B2 · kind B2 · utility

Assignee

• OMNIOME, INC. · US

Inventors

• Pinar Iyidogan · San Diego, US
• Mark C. Wallen · San Diego, US
• Ying Lin Liu · San Diego, US
• Kandaswamy Vijayan · San Diego, US

Key dates

• Filing date: Jan 9, 2018
• Grant date: Mar 24, 2020
• Expiry date: Mar 16, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are engineered DNA polymerases exhibiting modified functionality, and polynucleotides encoding same. Modified features include: (1) reduced catalytic activity in the presence of magnesium ions and/or (2) reduced affinity for primed template nucleic acid molecules in the absence of cognate nucleotide, and an ability to discriminate between cognate and non-cognate nucleotides under low salt conditions. Sequencing By Binding™ procedures employing the engineered polymerases have certain advantages. The engineered polymerases can have other uses as well.