Unfolding proximity probes and methods for the use thereof

US10597701B2 · kind B2 · utility

Assignee

• NAVINCI DIAGNOSTICS AB · SE

Inventors

• Ulf Landegren · Uppsala, SE
• Rachel Yuan Nong · Uppsala, SE
• Ola Söderberg · Österbybruk, SE
• Irene Helbing · Heidelberg, DE

Key dates

• Filing date: Dec 9, 2016
• Grant date: Mar 24, 2020
• Expiry date: Sep 20, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6876
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a proximity-probe based detection assay for detecting an analyte in a sample and in particular to a method that comprises the use of at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte directly or indirectly, wherein the nucleic acid domain of at least one of said proximity probes comprises a hairpin structure that can be unfolded by cleavage of the nucleic acid domain to generate at least one ligatable free end or region of complementarity to another nucleic acid molecule in said sample, wherein when the probes bind to said analyte unfolding said hairpin structure allows the nucleic acid domains of said at least first and second proximity probes to interact directly or indirectly.