Preparation of factor Xa derivatives

US10604748B2 · kind B2 · utility

Assignee

• Portola Pharmaceuticals, Inc. · US

Inventors

• Mark Karbarz · South San Francisco, US
• Pamela B. Conley · Palo Alto, US
• Genmin Lu · South San Francisco, US

Key dates

• Filing date: Jun 19, 2017
• Grant date: Mar 31, 2020
• Expiry date: Jun 19, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/22
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides methods for manufacturing a fXa derivative protein at large scale leading to high yield of highly pure protein product. The method may include adding a detergent to a sample that contains a polynucleotide construct encoding the protein and purifying the protein through a soybean trypsin inhibitor (STI)-based affinity chromatograph, an ion exchange and mixed mode chromatograph and a hydrophobic interaction.