Method for extracting differentiated cells
US10604770B2 · kind B2 · utility
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Key dates
| Filing date | Jul 16, 2015 |
| Grant date | Mar 31, 2020 |
| Priority date | — |
| Expiry date | Jul 16, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.
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