Real time cleavage assay
US10604793B2 · kind B2 · utility
2Cited by
76References
14Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 11, 2016 |
| Grant date | Mar 31, 2020 |
| Priority date | — |
| Expiry date | Nov 18, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.