Method for reconstructing aspergillus niger to increase citrate production
US10618940B2 · kind B2 · utility
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Key dates
| Filing date | Jan 11, 2018 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Feb 17, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/625
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention discloses a method for increasing citrate production from genome reconstructed Aspergillus niger. The method is to insert a gene of low affinity glucose transporter, LGT1, to genome of A. niger. The expression level of LGT1 is under control of promoter Pgas. The genome reconstructed A. niger is tolerant to higher fermentation temperature and lower pH than that of the parental strain. Moreover, the production, yield and purity of product from reconstructed A. niger are higher than that of parental strain, and the fermentation time is shorter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.