Prevention of N-terminal truncation in IgG light chains
US10618952B2 · kind B2 · utility
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Key dates
| Filing date | Oct 28, 2016 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Oct 28, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
We discovered that recombinant antibody light chains having a murine secretory leader sequence and an SYE motif at the N-terminus are truncated during post-translational processing. This disclosure provides two protein engineering solutions: to alter the SYE amino acid sequence of the Lc N-terminus to other alternatives; or to change the secretory leader peptide sequence. We have shown that both of these solutions are effective for preventing N-terminal light chain truncation.
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