Composition for reducing inhibition of nucleic acid amplification
US10619189B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 9, 2016 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Jan 12, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2527/125
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes a plurality of zirconium oxide particles, a non-ionic surfactant, and an organic iron-chelating reagent. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20° C. Optionally, the composition includes polyvinylpyrrolidone. Optionally, the composition comprises water. The composition has a pH of about 8.45 to 8.85. Methods of using the composition to prepare a sample for nucleic acid amplification are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.