Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

US10619204B2 · kind B2 · utility

Assignee

• Illumina Cambridge Limited · GB

Inventors

• Kevin L. Gunderson · San Diego, US
• Jingwei Bai · Beijing, CN
• Matthew KELLINGER · San Diego, US
• John M. Beierle · Laguna Niguel, US
• Jonathan Mark Boutell · Stanford-le-Hope, GB
• Roberto Rigatti · Saffron Walden, GB
• Maria Candelaria Rogert Bacigalupo · Encinitas, US
• Boyan Boyanov · Portland, US
• Klaus Maisinger · Witham, GB

Key dates

• Filing date: Nov 11, 2015
• Grant date: Apr 14, 2020
• Expiry date: Aug 2, 2036

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/0877
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments of the present disclosure relate to methods for capturing and amplifying target polynucleotides on a solid surface, in particular in a well in a microarray, wherein the microarray may comprise a) a substrate comprising at least one well, a surface surrounding the well and an inner well surface; b) a first layer covering the inner well surface and comprising at least one first capture primer pair; and c) a second layer covering the first layer and the surface surrounding the well.