Sequential sequencing
US10619206B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 10, 2017 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Mar 29, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/10
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Described herein are improved methods, compositions and kits for next generation sequencing (NGS). The methods, compositions and kits described herein enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (which can comprise regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information can be obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein can be useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.