Proximity assays using chemical ligation and hapten transfer
US10620217B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 2017 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Oct 9, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2440/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for in situ detecting proximity of two targets of interest featuring an antibody conjugated with a cleavable bridge component having a detectable moiety and an antibody conjugated with a non-cleavable bridge component. The bridge components each have a chemical ligation group adapted to form a covalent bond under particular conditions and when the targets are in close proximity. Following covalent bond formation, the cleavable bridge component can be cleaved from the antibody, effectively transferring the detectable moiety to the non-cleavable bridge component. Detection of the detectable moiety is indicative of the targets being in close proximity. The methods are compatible with both chromogenic and fluorogenic detection systems. The methods may be used to perform assays wherein one or more than one proximity event is detected on the same slide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.