Method and device for correcting level of expression of small RNA
US10622093B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 25, 2015 |
| Grant date | Apr 14, 2020 |
| Priority date | — |
| Expiry date | Nov 25, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/178
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of correcting an expression level(s) of a target small RNA(s) for comparative analysis of the expression level(s) in a plurality of samples includes adding at least one kind of standard substance to each of the plurality of samples, extracting nucleic acids from each sample to obtain a nucleic acid sample; measuring the amounts of the target small RNA(s) and the standard substance(s) present in each extracted nucleic acid sample; obtaining a representative value from the measured value(s) of the amount(s) of the standard substance(s) extracted; obtaining the difference or the ratio between a reference value arbitrarily set in connection with the amount(s) of the standard substance(s) extracted and the representative value of the standard substance(s) obtained for each sample; and correcting the expression level(s) of the target small RNA(s).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.