Methods and compositions of non-enzymatic amplification and direct detection of nucleic acids
US10626447B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2016 |
| Grant date | Apr 21, 2020 |
| Priority date | — |
| Expiry date | Jun 26, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56983
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The detection and quantification of nucleic acid sequences can be done using template catalyzed TARA transfer reactions without enzyme and PCR. It comes with the novel chemistry platform technology using Template Assisted Rapid Assay (TARA), an enzyme-free, PCR-less and rapid transfer reaction assay directly from samples from nasopharyngeal swab, nasal aspirate, oropharyngeal swab or blood. The procedures of the detection and quantification of nucleic acid sequences include utilizing two or more oligonucleotide probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive TARA reaction moieties. In addition, various methods, reagents, and kits for detecting and quantifying nucleic acid sequences and for determining the sequence of nucleic acids are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.