Methods and devices for digital PCR
US10626451B2 · kind B2 · utility
22Cited by
4References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 17, 2017 |
| Grant date | Apr 21, 2020 |
| Priority date | — |
| Expiry date | Feb 1, 2038 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0487
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology, may include at least partially segmenting a starting sampled into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.