Multi-pass sequencing

US10626455B2 · kind B2 · utility

Assignee

• BGI SHENZHEN · CN

Inventors

• Handong Li · San Jose, US
• Y. Tom Tang · Saratoga, US
• Jing Yu · Baofeng County, CN
• Hui Jiang · Lo Wu, CN
• Wenwei Zhang · Longbeilingcun, CN
• Guangyi Fan · Nanhu, CN
• He Fang Zhang · Beijing, CN
• Kailong Ma · Nanhu, CN
• Chunyu Geng · Nanhu, CN

Key dates

• Filing date: Nov 10, 2015
• Grant date: Apr 21, 2020
• Expiry date: May 1, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/48721
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.