Patent · US Active

Systems and methods for simultaneous acquisition of multiple planes with one or more chromatic lenses

US10627614B2 · kind B2 · utility

0Cited by
2References
20Claims
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Key dates

Filing dateMar 28, 2017
Grant dateApr 21, 2020
Priority date—
Expiry dateJan 17, 2038

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG02B21/0076
  • WIPO fieldOptics
  • WIPO sectorInstruments

Abstract

Systems and methods for fluorescent microscopy are disclosed where fluorophores can be excited over an excitation band to emit light in a wide emission band. Simultaneous acquisition of multiple planes in the sample can be achieved using a modified form of confocal microscopy. In one implementation, an objective employs a lens having optics exhibiting a large degree of axial chromatic aberration, such that emissions from different axially spaced focal planes are encoded by wavelength. Advantageously, simultaneous acquisition of multiple focal planes encoded by color can be processed to obtain efficient and rapid three-dimensional imaging of a sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.