Device and method for large volume transfection

US10633646B2 · kind B2 · utility

Assignee

• Lonza Cologne GmbH · DE

Inventors

• Ludger Altrogge · Pulheim, DE
• Timo Gleissner · Euskirchen, DE
• Andreas Heinze
• Sven Hermsmeier · Bonn, DE

Key dates

• Filing date: Apr 28, 2015
• Grant date: Apr 28, 2020
• Expiry date: Apr 28, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/87
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the el…