Methods and compositions for modulating AAV infection

US10633662B2 · kind B2 · utility

Assignees

• The Board of Trustees of the Leland Stanford Junior University · US
• Oregon Health & Science University · US

Inventors

• Sirika Pillay · Hayward, US
• Jan E. Carette · Palo Alto, US
• Michael Stewart Chapman · Lake Oswego, US
• Nancy Meyer · Portland, US
• Andreas Puschnik · Palo Alto, US
• Omar Davulcu · Portland, US

Key dates

• Filing date: Nov 9, 2016
• Grant date: Apr 28, 2020
• Expiry date: Nov 9, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14152
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods are provided for modulating adeno-associated virus (AAV) infection. For example, compositions and methods are provided for enhancing permissiveness of a target cell to AAV infection (e.g., by increasing levels of AAVR (KIAA0319L) in the cell), for reducing permissiveness of a target cell to AAV infection (e.g., by reducing levels of AAVR in the cell), and for nucleic acid delivery (e.g., by (i) increasing permissiveness of a target cell to AAV infection, e.g., by increasing the amount of AAVR in the cell; and (ii) contacting the target cell with an AAV particle that includes a nucleic acid of interest). Also provided are screening methods and kits for practicing the methods of the disclosure.