Patent · US Active

Synthetic oplophorus luciferases with enhanced light output

US10633690B2 · kind B2 · utility

4Cited by

4References

30Claims

0Family size

Assignee

Promega Corporation · US

Inventors

Lance P. Encell · Fitchburg, US
Mary Hall · Waunakee, US
Paul Otto · Madison, US
Gediminas Vidugiris · Fitchburg, US
Keith V. Wood · Verona, US
Monika G. Wood · Verona, US
Kristopher Zimmerman · Madison, US

Key dates

Filing date	Mar 6, 2019
Grant date	Apr 28, 2020
Priority date	—
Expiry date	Mar 6, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/61
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.