Systems, methods and components for isolating cells from a fluid sample

US10634587B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Hsian-Rong Tseng · Los Angeles, US
• Mitch A. Garcia · Malibu, US
• Min Song · Hwaseong-si, KR
• Libo Zhao · Tuji, CN
• Shuang Hou · Westlake Village, US
• Tom Tong LEE · San Diego, US

Key dates

• Filing date: Apr 9, 2018
• Grant date: Apr 28, 2020
• Expiry date: Apr 9, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2001/2886
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip.