Systems and methods for generating a structured illumination image
US10634892B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 29, 2017 |
| Grant date | Apr 28, 2020 |
| Priority date | — |
| Expiry date | Mar 17, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6478
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physical locations; moving the photomask or the sample to the determined physical locations in the randomized order under control of the control module and using a translation stage; illuminating the sample through the photomask using an excitation light; capturing a plurality of images at each of the physical locations using a sensor in order to generate a set of data points; using the randomized order to generate a composite image based on the set of data points and measuring the brightness of at least some of the data points; and adjusting the brightness of some of the set of data points.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.