Detection of DNA or RNA using single molecule arrays and other techniques

US10640814B2 · kind B2 · utility

Assignee

• QUANTERIX CORPORATION · US

Inventors

• David C. Duffy · Arlington, US
• Linan Song · Waltham, US
• Dandan Shan · Acton, US
• Mingwei Zhao · Qingdao, CN

Key dates

• Filing date: Mar 30, 2018
• Grant date: May 5, 2020
• Expiry date: Mar 31, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6837
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences.