Methods relating to the detection of recurrent and non-specific double strand breaks in the genome
US10640820B2 · kind B2 · utility
2Cited by
1References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 20, 2015 |
| Grant date | May 5, 2020 |
| Priority date | — |
| Expiry date | May 30, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/131
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Described herein are methods and systems relating to high throughput, genome-wide translocation sequencing (HTGTS) and/or detection of double-stranded DNA break (DSB) locations. The methods described herein can comprise generating DSBs in a nucleic acid sequence and performing nested PCR with the primers described herein. Described herein is an enhanced HTGTS approach. The assays and methods described herein permit the measurement of various DNA double-strand break (DSB) activities either intrinsic to the biological system or from outside agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.