Methods for visualization and quantification of fiber-like structures
US10641782B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 24, 2017 |
| Grant date | May 5, 2020 |
| Priority date | — |
| Expiry date | Jul 26, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30016
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Aspects of the present disclosure include a method for visualizing a fiber-like structure in a biological specimen, the method comprising: clearing the biological specimen comprising a fiber-like structure, wherein the fiber-like structure is detectably labeled; illuminating the cleared biological specimen with two light sheets from a first side and a second side to produce an image volume, wherein the second side is opposite to the first side and wherein the image volume comprises a representation of the fiber-like structure; defining a plurality of voxels within the representation of the fiber-like structure; processing each of the plurality of voxels to estimate a plurality of principal fiber-like structure orientations; and defining a starting point on the representation of the fiber-like structure and propagating a plurality of streamlines from the starting point, according to the plurality of principal fiber-like structure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.