Non-fluorescent imaging optical sectioning method and device based on annular off-axis illumination focal plane conjugation
US10642016B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 31, 2018 |
| Grant date | May 5, 2020 |
| Priority date | — |
| Expiry date | Dec 31, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/33
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
The invention discloses a non-fluorescent imaging optical sectioning method and device based on annular off-axis illumination focal plane conjugation. In an infinity-corrected optical system constituted by an objective lens with a large numerical aperture and a tube lens, off-axis light beams are used for bright-field microscopic imaging of a non-fluorescent sample; light beams emitted by M sub-sources which form an annularly distributed light source illuminate the sample off-axis at a large inclination angle; all sub-sources are lit to illuminate the sample at the same time, forming a superposed image of the sample illuminated by the M sub-sources individually on an image focal plane of the tube lens, and the camera shoots the image to obtain an optical-section image of a layer in the sample; and under the control of a translation mechanism, a sample stage allows different layers in the sample to coincide with an object focal plane of the objective lens to obtain optical-section images of multiple layers in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.