Methods for phototransfecting nucleic acids into live cells
US10646590B2 · kind B2 · utility
1Cited by
7References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2006 |
| Grant date | May 12, 2020 |
| Priority date | — |
| Expiry date | Dec 1, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.