L-type amylase variant and use thereof
US10647970B2 · kind B2 · utility
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9Claims
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Key dates
| Filing date | May 25, 2018 |
| Grant date | May 12, 2020 |
| Priority date | — |
| Expiry date | Jun 23, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses an L-type amylase variant and use thereof. The α-amylase variant is obtained by deleting the first N-terminal amino acid residue V from the α-amylase of B. licheniformis and replacing it with three other amino acid residues DGL. The α-amylase variant provided by the present invention has high catalytic activity under the acidic conditions of pH 5.0-5.8 and a high temperature of 100° C. or above. The acid resistance and thermal stability of these α-amylase variants are suitable for starch liquefaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.