Nucleic acid library generation methods and compositions
US10647981B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 8, 2016 |
| Grant date | May 12, 2020 |
| Priority date | — |
| Expiry date | Jun 17, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of forming a library of polynucleotide templates for DNA sequencing are disclosed. The methods include distributing a nucleic acid sample into different droplets in a set of emulsion droplets. The droplets into which the polynucleotide species are distributed include primers which binds to the polynucleotide species and are extended by a polymerase contained within the droplet. A set of extended primers are created simultaneously in a large number of droplets. An enriched set of extended primers are distributed into a second set of emulsion droplets containing a second primer which is extended by a polymerase contained within the second set of droplets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.