Methods for RNA analysis
US10648017B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 28, 2016 |
| Grant date | May 12, 2020 |
| Priority date | — |
| Expiry date | Nov 29, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8827
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5′ terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule. In particular, the invention concerns a method for determining the presence of a cap structure in an RNA molecule having a cleavage site for a catalytic nucleic acid molecule, a method for determining the capping degree of a population of RNA molecules having a cleavage site for a catalytic nucleic acid molecule, a method for determining the orientation of the cap structure in a capped RNA molecule having a cleavage site for a catalytic nucleic acid molecule and a method for determining relative amounts of correctly capped RNA molecules and reverse-capped RNA molecules in a population of RNA molecules, wherein the population comprises correctly capped and/or reverse-capped RNA molecules that have a cleavage site for a catalytic nucleic acid molecule. Moreover, the present invention provides uses of a catalytic nucleic acid molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.