Adherent cell culture method
US10655107B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 20, 2012 |
| Grant date | May 19, 2020 |
| Priority date | — |
| Expiry date | Sep 20, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M23/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are a method capable of evaluating adherent cells under an environment similar to an in vivo environment by a culture method similar to a two-dimensional culture, and applications thereof. An adherent cell culture method uses, as a culture chamber (10), a chamber in which two or more culture spaces each having an equivalent diameter (D) that is 1 to 5 times the diameter of a desired spheroid and each having a height (H) that is 0.3 to 5 times the equivalent diameter are arranged and a surface of each of the culture spaces has a water contact angle of 45 degrees or less. Spheroids of adherent cells are cultured in the respective culture spaces (11) arranged in the culture chamber (10).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.