Genomic sequence modification method for specifically converting nucleic acid bases of targeted DNA sequence, and molecular complex for use in same
US10655123B2 · kind B2 · utility
3Cited by
1References
6Claims
0Family size
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Key dates
| Filing date | Mar 4, 2015 |
| Grant date | May 19, 2020 |
| Priority date | — |
| Expiry date | Mar 4, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3513
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.