Methods, kits, and systems for multiplexed detection of target molecules and uses thereof

US10655163B2 · kind B2 · utility

Assignee

• The General Hospital Corporation · US

Inventors

• Ralph Weissleder · Peabody, US
• Sarit Agasti · Bengaluru, IN
• Vanessa M. Peterson · Somerville, US
• Adeeti V. Ullal · Mountain View, US

Key dates

• Filing date: Mar 4, 2019
• Grant date: May 19, 2020
• Expiry date: Mar 4, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Described herein are methods, compositions, kits and systems for multiplexed detection of target molecules from a sample. In some embodiments, the methods, compositions, kits and systems can be used to perform multiplexed protein analysis of a sample (e.g., a sample comprising a small number of cells or a single-cell sample). In some embodiments, the same sample subjected to a multiplexed protein analysis using the methods, compositions, kits and systems described herein can also be subjected to a nucleic acid (e.g., RNAs, microRNAs, and/or DNA) analysis, thereby creating an integrated expression profiling from a limited amount of sample.