Modified biotin-binding proteins for immobilization

US10655168B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Satwik Kamtekar · Mountain View, US
• Lubomir Sebo · Redwood City, US
• Leewin Chern · Fremont, US
• Thomas Linsky · Mountain View, US
• Jeremiah Hanes · Woodside, US
• Erik Miller · Seattle, US
• Ying Yang · Timonium, US
• Stephen Yue · Eugene, US

Key dates

• Filing date: Dec 21, 2018
• Grant date: May 19, 2020
• Expiry date: Dec 21, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2537/143
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions comprising covalently modified and mutated biotin-binding proteins, particularly biotin-binding proteins having a negative charge at physiological pH, are provided. Methods of producing such proteins are also provided, as are methods of immobilizing, sequencing, and making nucleic acids employing such proteins.