Nucleic acid constructs and methods of using the same

US10660970B2 · kind B2 · utility

Assignee

• GENEVANT SCIENCES GMBH · CH

Inventors

• Sean D. Monahan · Madison, US
• Michael S. DeClue · Seattle, US
• Pierrot Harvie · Bothell, US
• Russell N. JOHNSON · Seattle, US
• Amber E.E. Paschal · Redmond, US
• Mary G. Prieve · Lake Forest Park, US
• Debashish Roy · Seattle, US
• Charbel Diab · Tustin, US
• Michael E. Houston, JR. · Sammamish, US
• Anna Galperin · Seattle, US
• Maher QABAR · Sammamish, US

Key dates

• Filing date: Aug 16, 2019
• Grant date: May 26, 2020
• Expiry date: Aug 16, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y113/12007
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Described herein are block copolymers, and methods of making and utilizing such copolymers. The described block copolymers are disruptive of a cellular membrane, including an extracellular membrane, an intracellular membrane, a vesicle, an organelle, an endosome, a liposome, or a red blood cell. Preferably, in certain instances, the block copolymer disrupts the membrane and enters the intracellular environment. In specific examples, the block copolymer is endosomolytic and capable of delivering an oligonucleotide (e.g., an mRNA) to a cell. Compositions comprising a block copolymer and an oligonucleotide (e.g., an mRNA) are also disclosed.