Portable fluidic platform for rapid cell-free production of protein biologics

US10662457B1 · kind B1 · utility

Assignee

• Leidos, Inc. · US

Inventors

• John Dresios · Carlsbad, US
• Richard H. Griffey · Vista, US
• Challise J. Sullivan · San Diego, US
• Erik D. Pendleton · San Diego, US
• Henri Sasmor · San Diego, US
• William L. Hicks · Escondido, US
• Eric M. Amendt · San Diego, US

Key dates

• Filing date: Feb 16, 2018
• Grant date: May 26, 2020
• Expiry date: Jun 23, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y207/07007
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A portable fluidic platform for rapid and flexible end-to-end production of recombinant protein biologics includes a bioreactor system hosting stable and robust cell-free translation systems that is fluidically integrated with modular protein separation functionalities (e.g., size exclusion, ion exchange or affinity chromatography systems) for purification of the cell-free expressed product and which are configurable for process-specific isolation of different proteins, as well as for formulation. The bioreactor utilizes lysates from engineered eukaryotic (e.g., yeast) or prokaryotic (e.g., bacterial) strains that contain factors for protein folding and posttranslational modifications. Combination of various purification modules on the same fluidic platform allows flexibility of re-routing for purification of different proteins depending on specific target requirements. Protein synthesis and purification modules are integrated into self-contained disposable fluidic cartridge that eliminates cross-contamination between runs. The platform allows for flexible production of protein biologics within 24 hours (from DNA to purified product).