Polymer tagged nucleotides for single molecule electronic SNP assay

US10662463B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Youngjin Cho · New York, US
• Shiv Kumar · Montgomery, US
• Sergey Kalachikov · New York, US
• Chuanjuan Tao · Fort Lee, US
• Minchen Chien · Tenafly, US
• James J. Russo · New York, US

Key dates

• Filing date: Mar 22, 2016
• Grant date: May 26, 2020
• Expiry date: Mar 22, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/631
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention provides methods of using labeled nucleotide polyphosphate analogues to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, nucleotides and primer-conjugated nanopore proteins for use in such methods, and processes for producing such nucleotides and primer-conjugated nanopore proteins.