Method for extracting epsilon-polylysine and its hydrochloride salt from fermentation broth
US10669309B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 2017 |
| Grant date | Jun 2, 2020 |
| Priority date | — |
| Expiry date | Aug 15, 2037 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D2311/2699
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention provides a method for extracting ε-polylysine (ε-PL) and its hydrochloride salt from fermentation broth, which belongs to the field of bio-separation engineering. ε-PL and its hydrochloride salt are produced from fermentation broth through sequential solid-liquid separation, ultrafiltration, two-stage ion exchange, nanofiltration, evaporation concentration and drying techniques. Technologies of membrane filtration and two-stage ion exchange are applied to the preparation of ε-PL and its hydrochloride salt in the present invention, and the invention are characterized by reduced cost, improved automation, and increased product yield and purity, and the method of the present invention would be more suitable for industrial production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.