Dynamic range methods

US10669569B2 · kind B2 · utility

Assignee

• NAVINCI DIAGNOSTICS AB · SE

Inventors

• Mats Gullberg · Uppsala, SE
• Irene Weibrecht · Heidelberg, DE
• Carl-Magnus Clausson · Uppsala, SE
• Ola Söderberg · Österbybruk, SE

Key dates

• Filing date: Oct 14, 2011
• Grant date: Jun 2, 2020
• Expiry date: Dec 1, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/542
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for detecting and quantifying an analyte employ a pair of proximity probes, each comprising a proteinaceous target-binding domain coupled to a nucleic acid domain (NAD), which NADs interact when the proximity probes have bound in proximity to their respective target; and a set of markers, wherein each marker is a nucleic acid molecule comprising a binding domain and a reporter domain giving a detectable signal, can interact with said NADs to form a nucleic acid molecule from which a detectable signal is generated, or with a nucleic acid molecule generated by interaction of said NADs, cannot interact with said NADs simultaneously with another marker in the set, generates a signal that is distinguishable from another marker signal, and is present in an amount capable of detecting analyte at a range of concentrations differing from the range of concentrations detectable by other markers.