Method and systems for processing polynucleotides

US10669583B2 · kind B2 · utility

Assignee

• 10x Genomics, Inc. · US

Inventors

• Christopher Hindson · Pleasanton, US
• Michael Schnall-Levin · San Francisco, US
• Andrew D. Price · Hayward, US
• Paul Hardenbol · San Francisco, US
• Yuan Li · Dublin, US

Key dates

• Filing date: Apr 25, 2019
• Grant date: Jun 2, 2020
• Expiry date: Apr 25, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.