Antigenic polypeptides comprising pre-hairpin intermediate conformations of HIV-1 GP41

US10675346B2 · kind B2 · utility

Assignee

• Children's Medical Center Corporation · US

Inventors

• Bing Chen · Westwood, US
• Gary H. Frey · Nashua, US
• Jia Chen · Tainan, TW

Key dates

• Filing date: Nov 9, 2011
• Grant date: Jun 9, 2020
• Expiry date: Nov 22, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/162
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Isolated, antigenic polypeptides including a pre-hairpin intermediate conformation of gp41 and vectors encoding such polypeptides are provided. Exemplary pre-hairpin intermediate conformations of gp41 include an oligomerization domain; a heptad repeat 2 motif; and a membrane-proximal external region, where the polypeptide lacks a heptad repeat 1 motif, and where the isolated, antigenic polypeptides elicit production of a broadly neutralizing antibody against HIV when injected into a subject. Antibodies that bind to a pre-hairpin intermediate conformation of gp41 and methods of making antibodies a that bind to pre-hairpin intermediate conformation of gp41 are also provided. Vaccines against a pre-hairpin intermediate conformation of gp41, as well as methods of treating subjects infected with HIV, preventing HIV infection, and inhibiting HIV-mediated activities are also provided. Methods of screening compounds that bind to an isolated, pre-hairpin intermediate conformation of gp41 are further provided.