Compositions and methods for producing plants resistant to glyphosate herbicide

US10676754B2 · kind B2 · utility

Assignees

• E.I. DU PONT DE NEMOURS AND COMPANY · US
• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Vesna Djukanovic · Johnston, US
• Spencer Charles Jones · Des Moines, US
• Zhan-Bin Liu · West Chester, US
• Michael Lassner · Urbandale, US
• Leszek A. Lyznik · West Lafayette, US

Key dates

• Filing date: Jul 1, 2015
• Grant date: Jun 9, 2020
• Expiry date: Jan 21, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.